Case study 2 arroyo de montessus

Gloria Macapagal-Arroyo Gloria Macapagal-Arroyo born assumed presidency of the Philippines inafter a corruption scandal forced her predecessor, Joseph Estrada, from the post. Her move into Malacanang Palace, the presidential residence, served as a homecoming.

Case study 2 arroyo de montessus

Open in a separate window aExcept for three subjects noted immediately below footnote ball of the subjects in these studies were of recent European ancestry.

In logistic regressions of the bona fide case-control studies, these were considered as part of study 9b. All 65 ATM exons including the promoter region were PCR amplified and bidirectionally sequenced with the use of 64 primer pairs.

Two fragments were amplified and sequenced with the use of primers without the M13 tails. The cycling conditions were as follows: Cycle sequencing conditions were as follows: Sequence traces were aligned and analyzed with SeqScape v.

Full text of "Bulletin of the Pan American Union"

Sequences of the mutation-screening primers used are available from P. Mutation screening of the ATM gene at IARC study 9 was performed from whole-genome amplified WGA DNA with the use of a nested PCR strategy, followed by high-resolution melting curve analysis HRM analysis40,41 and then dye-terminator resequencing of samples that contained a melt curve aberration indicative of the presence of a sequence variant.

It thereby allows stratification of the samples according to their probe-target melting, i. Hence, the data analysis component of mutation scanning is performed separately on heterozygous and homozygous sample subsets.

The PCR consisted of 25 cycles of amplification with priming temperature and elongation time optimized for each amplicon multiplex. For the simultaneous mutation scanning and genotyping procedure, the same conditions were used, except that 1 a primer asymmetry ratio of 1: For an optimal efficiency of HRM, PCR2 amplicons were no longer than bp and amplified with 40 cycles for standard mutation scanning and 55 cycles for simultaneous mutation scanning and genotyping.

PCR2 products with melting curves that differed from the reference group were rearrayed onto new well plates and treated with exonuclease I and shrimp alkaline phosphatase for the removal of excess primers and nucleotide triphosphates exo-SAP treatment. Dye-terminator sequencing reactions BigDye Terminator, version1.

The resulting chromatograms were analyzed with the program Java SnpScreen. The software contains algorithms that spot potentially heterozygous positions on the basis of the joint data from the target sequence and each forward and reverse chromatogram pair.

Alternatively, the user can scan the superimposed chromatogram sets visually. After the screening has been completed, the program creates an output report that contains an amplicon-specific genotype for each sample screened.

All samples found to carry a rare sequence variant were reamplified from genomic DNA for confirmation of the presence of the variant.

DNAs from 13 kConFab breast cancer cases were mutation screened at both Regensburg and IARC, as were those of 30 other individuals that are part of another study being conducted by these centers.

[BINGSNIPMIX-3

The independently determined genotypes were identical for all 43 individuals. Results from the kConFab samples that were analyzed twice are included in the study 9 results. Statistical Methods To assess evidence of risk from the case-control frequency distribution of protein-truncating variants Tknown or very likely spliceogenic splice-junction variants SJand rare missense substitutions rMSswe constructed a single table with one entry per subject, zero or one rare sequence variant per subject, and annotations for study, case-control status, probability of being of recent African ancestry, and the estimated efficiency of mutation-screening method used.

For mutation-screening data extracted from the seven published case-control studies and 17 published case-only or control-only studies, our assumption of no more than one rare variant per subject was necessary because the studies pooled did not systematically report co-occurrence between rare variants.

Because the summed allele frequencies of the rare variants in these studies excluding the four that used the protein-truncation test [PTT only was about 4.

For subjects in our own mutation screening study who carried more than one rare variant, only the variant belonging to the most likely pathogenic grade was considered. We did not observe co-occurrence between any two rare variants of grade C35 or higher. Analyses of the bona fide case-control studies were adjusted for study site.

Case study 2 arroyo de montessus

The European and East Asian components of study 9 were treated as two separate studies, 9a and 9b, for this purpose. However, adjustment for study site was not possible for expanded analyses that included the case-only and control-only studies. For these subsidiary expanded analyses, we adjusted for ethnicity and mutation-screening methodology as described below.

The values were based on a recent review of mutation-screening methods 47 and were defined as follows: We considered that the PTT had a sensitivity of 0. Logistic regression trend tests were formatted such that subjects who did not carry any rare variant and carriers of the seven grades of rMSs C0, C15, C25, C35, C45, C55, and C65 defined by Align-GVGD 17 were assigned the default row labels 0,1,2,3,4,5,6, and 7, respectively.

These row labels were then used as a continuous variable in the logistic regressions. Thus, the same reference group of noncarriers was used for whole-gene analyses and domain-specific subanalyses. Post hoc power calculations were performed by specifying a hypothetical OR and population prevalence for each class of variant, together with the total probability of breast cancer prior to age The ORs that we specified for the individual grades of sequence variants, relative to C0 and the noncarriers, were as calculated from the whole-gene analysis for the grades for which there were reasonable numbers of observations: Because of the very low numbers of observations in C35 and C45, those ORs were set equal to C55 at 1.Millan, G., , New data about the geology of the Isle of Pines metamorphic complex, Cuba, Contributions to the Geology of Cuba: Special Publcation, v.

2: La Habana, Cuba, Instituto de Geológia y Paleontológia, Academia de Ciencias de Cuba, p. Download as PDF, TXT or read online from Scribd. Flag for inappropriate content.

Save. Space and Spatial Analysis in Archaeology - Free ebook download as PDF File .pdf), Text File .txt) or read book online for free.5/5(1).

In Colombia are available a discreet number of historical seismology investigations, dating back 50 years. This paper reviews basic information about earthquakes studies in Colombia, such as. Historical reports from the nineteenth century additionally mention an A.D.

eruption of Barú (Montessus de Ballore , ), and colonial era paintings further support this date. Abstract. The importance of the Lisbon earthquake is known worldwide not only among the scientific and technical communities but also in many other disciplines of human kind related to the effects and consequences of the earthquake.

Ecology and Society: Vol. 13, Iss. 2 ()